Nucleic Acid ProbesCRC Press, 31 mar. 1989 - 224 pagini With each chapter written by a distinguished expert in the biochemistry field, this comprehensive volume describes the preparation and use of a variety of radioactive and non-radioactive probes in situations ranging from research laboratories to routine diagnosis laboratories. The enzymatic and chemical techniques for labeling nucleic acid probes with radioisotopes and with non-radioactive ligands and haptens are discussed. Additionally, the associated methods for their detection, the use of these probes in the diagnosis of human and microbial pathogens, of plant viruses and viroids, and of human genetic disorders, as well as in the detection of nucleic acids in tissues and cells by in situ hybridization are presented. |
Cuprins
Chapter | 11 |
Chapter 2 | 11 |
Enzymatic Labeling of RNA | 18 |
Enzymatic Labeling of Oligonucleotides | 24 |
Preparation and Detection of Nonradioactive Nucleic Acid and Oligonucleotide | 33 |
Nucleic Acid Probes in the Diagnosis of Human Microbial Pathogens | 81 |
Chapter 4 | 113 |
Chapter 5 | 128 |
Detection of Specific DNA and RNA Sequences in Tissues and Cells by in situ | 139 |
Chapter 6 | 159 |
| 205 | |
Termeni și expresii frecvente
32P-labeled alkaline phosphatase alleles amplification analysis antibodies autoradiographic autosomal Biochem Biol biotin biotin-labeled biotinylated blot carrier cDNA cell chromosome Clin clinical cloned coli DNA polymerase containing deletion denatured detection disease disorders DNA fragments DNA hybridization DNA polymerase DNA probes DNA sequences DNase dNTPs dot-blot hybridization double-stranded DNA enzymatic Figure filter fluorescent gene genetic genome hepatitis high specific activity human hybridization assay hybridization probes infected intragenic isolated Klenow fragment labeled linkage M13 DNA markers method Microbiol Molecular molecules mutation Natl nick translation nitrocellulose nonradioactive nucleic acid hybridization nucleic acid probes Nucleic Acids Res nucleotides oligodeoxynucleotides oligonucleotide probes pathogens phage Photobiotin plant plasmid polymorphism prenatal diagnosis primer probes prepared procedure protein protocol reaction recombinant residues restriction enzyme RFLPs samples sandwich hybridization Section sensitivity single-stranded DNA specimens strand Symons synthesis target nucleic acid technique template tissue transcription transferase vector viral viroid Virol virus viruses vitro X chromosome
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Nanoengineered Nanofibrous Materials Selcuk Guceri,Yury G. Gogotsi,Vladimir Kuznetsov Previzualizare limitată - 2007 |