Analytical Techniques in ImmunochemistryCRC Press, 23 dec. 1991 - 364 pagini Explores the principles, and presents step-by-step recipes for isolating and measuring biological materials using modern immunochemical methods, including such new procedures as high-performance immunoaffinity chromatography, capillary electrophoresis, and blotting. Also includes clear drawings of |
Cuprins
SIZE EXCLUSION CHROMATOGRAPHY | 3 |
Low and MediumPressure Techniques | 12 |
References | 20 |
Chromatographic Equipment | 27 |
References | 38 |
334 | 71 |
IMMUNOAFFINITY CHROMATOGRAPHY | 75 |
POLYACRYLAMIDE GEL ELECTROPHORESIS | 103 |
CHROMATOFOCUSING | 177 |
GEL PRECIPITATION TECHNIQUES | 191 |
ELUTION AND BLOTTING TECHNIQUES | 211 |
GEL STAINING TECHNIQUES | 235 |
IMMUNOASSAYS | 263 |
PROTEIN MEASUREMENT | 301 |
ANTIBODY DIGESTION TECHNIQUES | 324 |
| 333 | |
Termeni și expresii frecvente
0.01 M phosphate 30 minutes acrylamide affinity chromatography agarose allow ammonium ammonium persulfate ampholyte Anal antibody antigen apparatus applied assay attached avidin beaker binding Biochem BioRad blot cassette cell chemical chromatofocusing Chromatogr column described dilution Dissolve distilled water double-distilled water electrode elution buffer enzyme Figure Fill film filter paper fractions gently glass plate gradient hours at room HPIAC HPLC immobilized immunoaffinity immunoaffinity chromatography immunoassays immunoelectrophoresis incubate ion-exchange isoelectric focusing isolated labeled lectin ligand material matrix membrane methanol minutes at room mixture molecules nitrocellulose nucleic acids overhead mixer packing performed Pharmacia phosphate buffer pipet Place the gel polyacrylamide gels precipitin Prepare procedure proteins pump reaction reagent Remove the gel reservoir room temperature separation Sephadex serum sheet silane slab gel sodium chloride sodium hydroxide solution spacer staining supernatant surface syringe tank technique test sample transfer tube gel vacuum wash
Pasaje populare
Pagina 322 - Continuous cultures of fused cells secreting antibody of predefined specificity," Nature (London) 256 (1975) 495-497.
Pagina 322 - K: A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines.
Referințe la această carte
Encyclopedia of Chromatography 2004 Update Supplement Jack Cazes Nu există previzualizare disponibilă - 2004 |