Handbook of Detection of Enzymes on Electrophoretic GelsCRC Press, 26 dec. 2002 - 568 pagini Still widely used as gene markers, isozymes detected by zymogram techniques have proven valuable in a range of other biological applications over the last few years. Along with these new applications, many new techniques have also emerged. Yet more than eight years since the Handbook of Detection of Enzymes on Electrophoretic Gels was first publish |
Cuprins
Methods of Detection of Specific Enzymes | |
Minimal Medium for Escherichia coli | |
Citrate Medium for Pediococcus cerevisiae | |
Alphabetical List of Enzymes | |
Buffer Systems Used for Enzyme Electrophoresis | |
Alte ediții - Afișează-le pe toate
Handbook of Detection of Enzymes on Electrophoretic Gels Gennady P. Manchenko Previzualizare limitată - 2002 |
Handbook of Detection of Enzymes on Electrophoretic Gels Gennady P. Manchenko Vizualizare fragmente - 1994 |
Handbook of Detection of Enzymes on Electrophoretic Gels Gennady P. Manchenko Nu există previzualizare disponibilă - 2019 |
Termeni și expresii frecvente
37°C until dark Academic Press acetate acid activity bands additional agar agarose Anal Apply areas background Biochem blue bands appear catalyzed cell cellulose acetate color Comments containing coupled dark at 37°C dark blue bands dehydrogenase detection developed Dimer electrophoresis electrophoretic gels Enzyme Nomenclature ENZYME SOURCE Bacteria ethanol filter paper fluorescent fungi gel electrophoresis gel surface Genet glucose Glucose-6-phosphate human included Incubate the gel indicator invertebrates isozymes kinase linking localization membrane Method Method Visualization Scheme mg/ml mixture ml Procedure NADH NADP NAMES NC-IUBMB Notes obtained overlay oxidase phosphate buffer photograph plants polyacrylamide gel prepared Procedure protein REACTION reduced References remove room temperature salt San Diego sensitive sodium specific Stage stained gel staining solution Staining Solution1 substrate tion Tris-HCl buffer U/ml vertebrates SUBUNIT STRUCTURE visible Visualization Scheme Wash zymogram