Handbook of BioseparationsSatinder Ahuja Elsevier, 23 iun. 2000 - 722 pagini It is generally recognized that the commercial success of biotechnology products is highly dependent on the successful development and application of high-powered separation and purification methods. In this practical and authoritative handbook, the separation of proteins, nucleic acids, and oligonucleotides from biological matrices is covered from analytical to process scales. Also included in a chapter on the separation of monoclonal antibodies, which have found numerous uses as therapeutic and diagnostic agents. Analytical techniques include an interesting montage of chromatographic methods, capillary electrophoresis, isoelectric focusing, and mass spectrometry. Among separation and purification methods, liquid-liquid distribution, displacement chromatography, expanded bed adsorption, membrane chromatography, and simulated moving bed chromatography are covered at length. Regulatory and economic considerations are addressed, as are plant and process equipment and engineering process control. A chapter on future developments highlights the application of DNA chip arrays as well as evolving methodologies for a large number of drugs that are under development for treatment of cancer, AIDS, rheumatoid arthritis, and Alzheimer's disease. Handbook of Bioseparations serves as an essential reference and guidebook for separation scientists working in the pharmaceutical and biotechnology industries, academia, and government laboratories. Key Features * Covers bioseparations of proteins, nucleic acids, and monoclonal antibodies * Encompasses both analytical and process-scale methods * Elucidates the importance of engineering process control * Details selection of plant and process equipment * Addresses economic considerations * Discusses future developments |
Cuprins
| 23 | |
Current Status and Challenges for the Future | 71 |
Chapter 4 Capillary Electrophoresis of Compounds of Biological Interest | 237 |
Chapter 5 Isoelectric Focusing | 263 |
Chapter 6 Mass Spectrometry of Biomolecules | 299 |
Chapter 7 LiquidLiquid Partitioning Methods for Bioseparations | 329 |
Chapter 8 Separation of Nucleic Acids and Proteins | 365 |
Chapter 9 Bioseparations by Displacement Chromatography | 379 |
Chapter 12 Adsorptive Membranes for Bioseparations | 453 |
Chapter 13 Simulated MovingBed Chromatography for Biomolecules | 475 |
Chapter 14 LargeScale Chromatographic Purification of Oligonucleotides | 511 |
Chapter 15 Separation of Antibodies by Liquid Chromatography | 535 |
Chapter 16 Processing Plants and Equipment | 633 |
Chapter 17 Engineering Process Control of Bioseparation Processes | 659 |
Chapter 18 Economics of Bioseparation Processes | 667 |
Chapter 19 Future Developments | 687 |
Chapter 10 Physicochemical Basis of ExpandedBed Adsorption for Protein Purification | 417 |
Chapter 11 ExpandedBed Adsorption Process for Protein Capture | 431 |
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Termeni și expresii frecvente
achieved acid addition adsorbent adsorption affinity analysis analytical antibodies application approach associated behavior binding biological bioseparation buffer capacity capillary cell cell culture charge chemical Chromatogr chromatography column complex concentration constant containing cost dependencies described detection displacement effects electrophoresis elution example exchange expression extraction factor FIGURE first flow focusing fraction gradient groups HPLC human hydrophobic important impurities increase interaction involved ligand liquid mass material membrane methods mixture mobile mode molecular molecules monoclonal obtained oligonucleotide operation particles peptide performed phase polypeptide or protein polypeptides preparative present procedures properties protein purification purity range recombinant recovery relatively removal resolution salt sample scale selectivity separation solid solution solvent sorbent specific step structure studies surface Table techniques temperature tion values volume weight Western blot
Referințe la această carte
Handbook of Isoelectric Focusing and Proteomics David Garfin,Satinder Ahuja Previzualizare limitată - 2005 |