Molecular Biology and Genetic Engineering of YeastsCRC Press, 27 dec. 1991 - 416 pagini Molecular Biology and Genetic Engineering of Yeasts presents a comprehensive examination of how yeasts are used in genetic engineering. The book discusses baker's yeast, in addition to a number of unconventional yeasts being used in an increasing number of studies. 175 figures help illustrate the information presented. Topics discussed include yeast transformation, yeast plasmids, protein localization and processing in yeast, protein secretion, various aspects of Saccharomyces cerevisiae, and heterologous expression and secretion. |
Cuprins
Yeast Transformation | 11 |
III | 11 |
IV | 17 |
V | 22 |
IX | 27 |
References | 28 |
Chapter | 41 |
VI | 50 |
Evolutionary Significance | 208 |
Chapter | 215 |
Heterologous Expression of Genes Coding for Peroxisomal Proteins | 222 |
Use in Biotechnology | 228 |
Transmembrane Integration Signals | 234 |
Prospects | 241 |
Thermosensitive sec Mutants | 245 |
V | 253 |
Linear and Circular Plasmids from Kluyveromyces | 57 |
II | 66 |
III | 73 |
XI | 81 |
References | 87 |
2 | 93 |
d | 101 |
Basal Promoter Sequences | 118 |
Regulatory Proteins | 125 |
References | 148 |
Chapter 5 | 161 |
Role of the GAL3 Protein | 167 |
References | 169 |
Chapter | 175 |
Chapter | 183 |
VII | 189 |
Chapter | 195 |
In Vitro Translocation Systems | 261 |
Chapter | 279 |
Chapter | 287 |
Saccharomyces cerevisiae Invertase | 293 |
Effects on Secretion of Alterations in the Signal Sequence and Mature | 296 |
In Vitro Studies of Translocation into the Endoplasmic Reticulum | 302 |
Chapter 9 | 309 |
In Vitro Study of Protein Degradation | 316 |
Other Ubiquitin Functions | 323 |
Chapter 10 | 329 |
C | 333 |
Limitations to Heterologous Gene Expression | 340 |
4 | 360 |
XI | 374 |
| 385 | |
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Termeni și expresii frecvente
ABF1 Acad activation domain amino acids analysis binding domain Biol Cell centromere chromosome cloned coding coli complex consensus containing copy number CYC1 CYC7 CYCI cytochrome cytoplasm deletion dimer DNA sequences DNA-binding domain EMBO encoding enzyme eukaryotic factor Figure fragment function fusion galactose GCN4 Genet genome glucose Golgi HAP1 HAP2 HIS3 homology hybrid initiation inserted interaction invertase isolated killer Kingsman Kluyveromyces lactis LEU2 linear located mammalian membrane mitochondrial mRNA mutants Natl Nature London nuclear Nucleic Acids Nucleic Acids Res nucleotide peptide permission peroxisomes plasmid precursor presequence Proc promoter Ptashne recombination region regulatory proteins REP1 repeats replication repression residues RNA polymerase RNA polymerase II Saccharomyces cerevisiae Schizosaccharomyces pombe signal specific strain structure subunit synthesis target TATA box telomeres terminal TFIID toxin transcriptional activator transformation translocation transposition Ty element UAS1 UAS2 UASS URA3 vacuolar vector vitro vivo wild-type yeast zinc finger