Léveillé added 87 species, making a total of 246 species. The fungi of Sumatra, Borneo, and other islands are partly the same and partly allied, but of a similar tropical character.

The fungi of the island of Ceylon, collected by Gardner, Thwaites, and König, were numerous. The Agarics comprise 302 species, closely resembling those of our own country.* It is singular that every one of the subgenera of Fries is represented, though the number of species in one or two is greatly predominant. Lepiota and Psalliota alone comprise one-third of the species, while Pholiota offers only a single obscure species. The enumeration recently published of the succeeding families contains many species of interest.

In Africa, the best explored country is Algeria, although unfortunately the flora was never completed. The correspondence between the fungi of Algeria and European countries is very striking, and the impression is not removed by the presence of a few sub-tropical forms. It is probable that were the fungi of Spain known the resemblance would be more complete.

From the Cape of Good Hope and Natal collections have been made by Zeyher,‡ Drége, and others, and from these we are enabled to form a tolerable estimate of the mycologic flora. Of the Hymenomycetes, the greater part belong to Agaricus; there are but four or five Polypori in Zeyher's collection, one of which is protean. The Gasteromycetes are interesting, belonging to many genera, and presenting two, Scoleciocarpus and Phellorinia, which were founded upon specimens in this collection. Batarrea, Tulostoma, and Mycenastrum are represented by European species. There are also two species of Lycoperdon, and one of Podaxon. Besides these, there is the curious Secotium Gueinzii. The genus Geaster does not appear in the collection, nor Scleroderma. Altogether the Cape flora is a pcculiar one, and can scarcely be compared with any other.

At the most, only scattered and isolated specimens have been

*Berkeley and Broome, "Fungi of Ceylon," in "Journ. Linn. Soc." for May, 1871.

+ "Flore d'Algerie, Cryptogames" (1846, &c.).

Berkeley, in Hooker's "Journal of Botany," vol. ii. (1843), p. 408.

recorded from Senegal, from Egypt, or from other parts of Africa, so that, with the above exceptions, the continent may be regarded as unknown.

From this imperfect summary it will be seen that no general scheme of geographical distribution of fungi can as yet be attempted, and the most we can hope to do is to compare collection with collection, and what we know of one country with what we know of another, and note differences and agreements, so as to estimate the probable character of the fungi of other countries of which we are still in ignorance. It is well sometimes that we should attempt a task like the present, since we then learn how much there is to be known, and how much good work lies waiting to be done by the capable and willing hands that may hereafter undertake it.

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XIV.

COLLECTION AND PRESERVATION.

THE multitudinous forms which fungi assume, the differences of substance, and variability in size, render a somewhat detailed account of the modes adopted for their collection and preserva

tion necessary. been indicated, so that there need be no difficulty in selecting the most suitable spots, and as to the period of the year, this will be determined by the class of objects sought. Although it may be said that no time, except when the ground is covered with snow, is entirely barren of fungi, yet there are periods more prolific than others.* Fleshy fungi, such as the Hymenomycetes, are most common from September until the frosts set in, whereas many microscopic species may be found in early spring, and increase in number until the autumn.

The habitats of the various groups have already

The collector may be provided with an ordinary collecting box, but for the Agarics an open shallow basket is preferable. A great number of the woody kinds may be carried in the coatpocket, and foliicolous species placed between the leaves of a pocket-book. It is a good plan to be provided with a quantity of soft bibulous paper, in which specimens can be wrapped when collected, and this will materially assist in their preservation when transferred to box or basket. A large clasp-knife, a small pocket-saw, and a pocket-lens will complete the outfit for ordinary occasions. In order to preserve the fleshy fungi for the herbarium, there is but one method, which has often been described. *The genus Chionyphe occurs on granaries under snow, as well as in that formidable disease, the Madura fungus-foot. (See Carter's " Mycetoma.")

The Agaric, or other similar fungus, is cut perpendicularly from the pileus downwards through the stem. A second cut in the

same direction removes a thin slice, which represents a section of the fungus; this may be laid on blotting paper, or plant-drying paper, and put under slight pressure to dry. From one-half of the fungus the pileus is removed, and with a sharp knife the gills and fleshy portion of the pileus are cut away. In the same manner the inner flesh of the half stem is also cleared. When dried, the half of the pileus is placed in its natural position on the top of the half stem, and thus a portrait of the growing fungus is secured, whilst the section shows the arrangement of the hymenium and the character of the stem. The other half of the pileus may be placed, gills downward, on a piece of black paper, and allowed to rest there during the night. In the morning the spores will have been thrown down upon the paper, which may be placed with the other portions. When dry, the section, profile, and spore paper may be mounted together on a piece of stiff paper, and the name, locality, and date inscribed below, with any additional particulars. It is advisable here to caution the collector never to omit writing down these particulars at once when the preparations are made, and to place them together, between the folds of the drying paper, in order to prevent the possibility of a mistake. Some small species may be dried whole or only cut down the centre, but the spores should never be forgotten. When dried, either before or after mounting, the specimens should be poisoned, in order to preserve them from the attacks of insects. The best medium for this purpose is carbolic acid, laid on with a small hog-hair brush. Whatever substance is used, it must not be forgotten by the manipulator that he is dealing with poison, and must exercise caution. If the specimens are afterwards found to be insufficiently poisoned, or that minute insects are present in the herbarium, fresh poisoning will be necessary. Some think that benzine or spirits of camphor is sufficient, but as either is volatile, it is not to be trusted as a permanent preservative. Mr. English, of Epping, by an ingenious method of his own, preserves a great number of the fleshy species in their natural position, and although

valueless for an herbarium, they are not only very ornamental, but useful, if space can be devoted to them.

Leaf parasites, whether on living or dead leaves, may be dried in the usual way for drying plants, between folds of bibulous paper under pressure. It may be sometimes necessary with dead leaves to throw them in water, in order that they may be flattened without breaking, and then dry them in the same manner as green leaves. All species produced on a hard matrix, as wood, bark, etc., should have as much as possible of the matrix pared away, so that the specimens may lie flat in the herbarium. This is often facilitated in corticolous species by removing the bark and drying it under pressure.

The dusty Gasteromycetes are troublesome, especially the minute species, and if mounted openly on paper are soon spoiled. A good plan is to provide small square or round cardboard boxes, of not more than a quarter of an inch in depth, and to glue the specimen to the bottom at once, allowing it to dry in that position before replacing the cover. The same method should be adopted for many of the moulds, such as Polyactis, etc., which, under any circumstances, are difficult to preserve.

In collecting moulds, we have found it an excellent plan to go out provided with small wooden boxes, corked at top and bottom, such as entomologists use, and some common pins. When a delicate mould is collected on a decayed Agaric, or any other matrix, after clearing away with a penknife all unnecessary portions of the matrix, the specimen may be pinned down to the cork in one of these boxes. Another method, and one advisable also for the Myxogastres, is to carry two or three pill-boxes, in which, after being wrapped in tissue paper, the specimen may be placed.

A great difficulty is often experienced with microscopic fungi, such, for instance, as the Sphæriacei, in the necessity, whenever a new examination is required, to soak the specimen for some hours, and then transfer the fruit to a slide, before it can be compared with any newly-found specimen that has to be identified. To avoid this, mounted specimens ready for the microscope

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