Brain Microtuble Associated ProteinsJesus Avila, R. Brandt, K. S. Kosik CRC Press, 28 oct. 1997 - 353 pagini Brain Microtubule Associated Proteins lays a firm foundation for understanding the brain microtubule associated proteins and how they contribute to the neurodegeneration of Alzheimer's disease. Few other reference works contain within a single source such a complete treatment of this topic. The authors cast a broad net over the field by devoting the first four chapters to each of the major brain microtubule associated proteins, MAP1A, MAP1B, MAP2 and tau. Completing this background material are chapters on the cellular role of these proteins in developing neurons and the sorting of tau to axons. The remaining chapters focus on tau - its kinases, its phosphatases, and other post-translational modifications such as ubiquitination and glycation. The second part of the book takes a more practical approach and includes chapters on the purification of microtubule associated proteins, functional assays using gene-transfer experiments, immunofluorescence and video microscopic analysis. This book is intended not only to be a reference source for expert laboratories but also to provide information which will allow a novice in the field to choose the best method for a particular purpose. |
Cuprins
A key regulator | 17 |
Possible roles for MAP2 in neuronal pathology | 33 |
MAPS in growth cones | 53 |
Tau protein kinases | 73 |
Tau phosphatases | 95 |
Ubiquitination of tau as an abnormal protein | 113 |
Sorting of tau | 153 |
Modelling prionlike processing of tau protein in Alzheimers disease | 185 |
Expression and purification of tau for in vitro studies | 245 |
Immunofluorescence population assays for studying MAP function | 259 |
Using video microscopy to study taus effects on the dynamic | 275 |
Expression of MAP cDNAs in eukaryotic cells | 293 |
Analysis of the phosphorylation state of tau in situ | 313 |
Biochemical isolation and characterization of paired helical filaments | 331 |
Finding biomedical information on the web | 363 |
Termeni și expresii frecvente
abnormally phosphorylated Acad actin activity aggregation Alzheimer's disease amino acid antibodies assay associated protein Avila axons Biernat Biochem Brain Res buffer CDK5 Cell Biol Chem concentration coverslip culture cytoskeleton dendrites diabetes epitope expression extracellular FEBS Lett Figure formation fraction fragment function glucose glycation Goedert growth cones Grundke-Iqbal heavy chain Hirokawa hyperphosphorylation Ihara immunoreactivity incubation inhibition interaction Iqbal isoforms Kirschner Kosik Ledesma light chain lysine Mandelkow MAP1 proteins MAP1A MAP1B MAP2 Matus microtubule assembly microtubule binding microtubule-associated protein microtubule-associated protein tau molecular mRNA N-terminal Natl neurite Neurochem neurofibrillary tangles neuronal cell neurons Neurosci normal tau paired helical filaments pathology peptide PHF-tau phosphatases phosphorylated tau phosphorylation phosphorylation of tau polyprotein Proc proteases protein kinase protocol purified region repeat domain residues sequence soluble stability structure subunit tau isoforms tau molecule tau mRNA tau phosphorylation tau protein tau-tau transfected tubulin ubiquitin vitro vivo Wischik