Functional Organization of The Nucleus: A Laboratory Guide

Acad acetate active aliquots allele analysis antigens assay binding Biochem Biology buffer Cell Biol Cell Cambridge centrifuge centromere chromatin chromatin structure chromosomal proteins cloned complex components concentration containing coverslip denaturation described detection digestion diluted DNA sequences DNase Drosophila melanogaster EDTA eggs embryos enzyme ethanol euchromatin exonuclease extract factors filter fixation fluorescence fraction fragments function gene genetic genome heterochromatin histone histone H3 hybridization Incubate interphase isolated labeled lethal loci metaphase method mg/ml MgCl2 micrococcal nuclease microscope minichromosomes minutes mitotic molecular monoclonal antibodies mRNA mutations NaCl Natl nuclear envelope nuclear proteins nuclei nucleosome nucleosome assembly oligonucleotide pellet pg/ml phenotype plasmid polytene chromosomes preparation probe Proc procedure protein protocol purified reaction reagents region remove replication resuspended Rinse RNA polymerase room temperature signal slides sodium solution specific spermatocytes staining Su(var SUEHGR supernatant technique template tissue transcription tube variegating vitro vivo wash wild-type Xenopus yeast